We work on molecular mechanism of bacterial RNA polymerase and its regulation by accessory proteins, small ligands, nucleic acid signals, and antibiotics. Several projects are available; examples include (i) identification of cellular partners of a virulence factor RfaH by in vivo crosslinking; (ii) studies of the mechanism of RfaH re-folding in a tube; (iii) dissection of RNA polymerase interactions with several transcription factors by genetic, biochemical, and biophysical approaches.
Five Recent Publications:
- Deaconescu AM, Sevostyanova A, Artsimovitch I & Grigorieff N. (2012) NER machinery recruitment by the transcription-repair coupling factor involves unmasking of a conserved intramolecular interface. Proc Natl Acad Sci U S A. in press
- Furman R, Sevostyanova A & Artsimovitch I. (2011) Transcription initiation factor DksA has diverse effects on RNA chain elongation. Nucleic Acids Res. doi:10.1093/nar/gkr1273.
- Artsimovitch I, Svetlov V, Nemetski SM, Epshtein V, Cardozo T & Nudler E. (2011) Tagetitoxin inhibits RNA polymerase through trapping of the trigger loop. J Biol Chem. 286, 40395-400.
- Sevostyanova A, Belogurov GA, Mooney RA, Landick R & Artsimovitch I. (2011) The beta subunit gate loop is required for RNA polymerase modification by RfaH and NusG. Mol Cell. 43, 253-62.
- Santangelo TJ & Artsimovitch I. (2011) Termination and antitermination: RNA polymerase runs a stop sign. Nat Rev Microbiol. 9, 319-29.
Publication at PubMed